9.3 proc survey procedures Search Results


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FAP affinity of different fluorescent imaging compounds. IC 50 calculations each of the FTF conjugates. Recombinant murine FAP activity was measured using the fluorogenic FAP substrate <t>Z-Gly-Pro-AMC.</t> Concentrations of FTF spanned from 5 pM to 250 nM and are plotted on a logarithmic axis. Relative activity ( y axis) was scaled to measurements from wells with (1) FAP and Z-Gly-Pro-AMC but no inhibitor, and (2) Z-Gly-Pro-AMC but no FAP or inhibitor. IC 50 (expressed as nM concentration in the graphs) was determined from a nonlinear, three-parameter, inhibitor vs response curve fit.
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FAP affinity of different fluorescent imaging compounds. IC 50 calculations each of the FTF conjugates. Recombinant murine FAP activity was measured using the fluorogenic FAP substrate <t>Z-Gly-Pro-AMC.</t> Concentrations of FTF spanned from 5 pM to 250 nM and are plotted on a logarithmic axis. Relative activity ( y axis) was scaled to measurements from wells with (1) FAP and Z-Gly-Pro-AMC but no inhibitor, and (2) Z-Gly-Pro-AMC but no FAP or inhibitor. IC 50 (expressed as nM concentration in the graphs) was determined from a nonlinear, three-parameter, inhibitor vs response curve fit.
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FAP affinity of different fluorescent imaging compounds. IC 50 calculations each of the FTF conjugates. Recombinant murine FAP activity was measured using the fluorogenic FAP substrate <t>Z-Gly-Pro-AMC.</t> Concentrations of FTF spanned from 5 pM to 250 nM and are plotted on a logarithmic axis. Relative activity ( y axis) was scaled to measurements from wells with (1) FAP and Z-Gly-Pro-AMC but no inhibitor, and (2) Z-Gly-Pro-AMC but no FAP or inhibitor. IC 50 (expressed as nM concentration in the graphs) was determined from a nonlinear, three-parameter, inhibitor vs response curve fit.
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FAP affinity of different fluorescent imaging compounds. IC 50 calculations each of the FTF conjugates. Recombinant murine FAP activity was measured using the fluorogenic FAP substrate <t>Z-Gly-Pro-AMC.</t> Concentrations of FTF spanned from 5 pM to 250 nM and are plotted on a logarithmic axis. Relative activity ( y axis) was scaled to measurements from wells with (1) FAP and Z-Gly-Pro-AMC but no inhibitor, and (2) Z-Gly-Pro-AMC but no FAP or inhibitor. IC 50 (expressed as nM concentration in the graphs) was determined from a nonlinear, three-parameter, inhibitor vs response curve fit.
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Image Search Results


FAP affinity of different fluorescent imaging compounds. IC 50 calculations each of the FTF conjugates. Recombinant murine FAP activity was measured using the fluorogenic FAP substrate Z-Gly-Pro-AMC. Concentrations of FTF spanned from 5 pM to 250 nM and are plotted on a logarithmic axis. Relative activity ( y axis) was scaled to measurements from wells with (1) FAP and Z-Gly-Pro-AMC but no inhibitor, and (2) Z-Gly-Pro-AMC but no FAP or inhibitor. IC 50 (expressed as nM concentration in the graphs) was determined from a nonlinear, three-parameter, inhibitor vs response curve fit.

Journal: Bioconjugate Chemistry

Article Title: FAP-Targeted Fluorescent Imaging Agents to Study Cancer-Associated Fibroblasts In Vivo

doi: 10.1021/acs.bioconjchem.4c00426

Figure Lengend Snippet: FAP affinity of different fluorescent imaging compounds. IC 50 calculations each of the FTF conjugates. Recombinant murine FAP activity was measured using the fluorogenic FAP substrate Z-Gly-Pro-AMC. Concentrations of FTF spanned from 5 pM to 250 nM and are plotted on a logarithmic axis. Relative activity ( y axis) was scaled to measurements from wells with (1) FAP and Z-Gly-Pro-AMC but no inhibitor, and (2) Z-Gly-Pro-AMC but no FAP or inhibitor. IC 50 (expressed as nM concentration in the graphs) was determined from a nonlinear, three-parameter, inhibitor vs response curve fit.

Article Snippet: The inhibitory effect of FTF compounds was measured using the fluorogenic FAP substrate, Z-Gly-Pro-AMC (MedChemExpress).

Techniques: Imaging, Recombinant, Activity Assay, Concentration Assay